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KMID : 1094720130180050981
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Biotechnology and Bioprocess Engineering
2013 Volume.18 No. 5 p.981 ~ p.988
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Recovery of native protein from potato root water by expanded bed adsorption with amberlite XAD7HP
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Zeng Fan-Kui
Liu Hong
Ma Peng-Jun
Liu Gang
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Abstract
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Potato root water (PRW) contains ∼1.5% protein. In this study, expanded bed adsorption (EBA) chromatography with Amberlite XAD7HP resin adsorbent was used to isolate native protein from crude PRW. The optimal pH and ionic strength for potato protein binding onto Amberlite XAD7HP were 5.0 and 20 mmol/L. The EBA-refined proteins were dried by vacuum freeze drying and spray drying at varying outlet temperatures. Results indicated that low temperature spray drying was the most cost effective method with respect to retaining protease inhibitor activities. The dried protein concentrates appeared bright yellow or dark reddish brown, with a total glycoalkaloid content of ∼170 μg/g. The protease inhibitor activity was ∼400 mg/g and 11 ∼ 12 mg/g for trypsin inhibition and chymotrypsin inhibition, respectively. The results presented here suggest that EBA using Amberlite XAD7HP as the adsorbent is a feasible strategy for the direct adsorption of native protein from crude PRW.
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KEYWORD
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potato root water, potato protein, protease inhibitors, expanded bed adsorption, Amberlite XAD7HP
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